peroxidase-antiperoxidase technique
- peroxidase-antiperoxidase technique
- a technique for detecting antigen or antibody in tissue sections. The tissue section is incubated with rabbit antibody specific for the antigen to be detected, followed by an excess of antirabbit IgG. A complex of horseradish peroxidase and rabbit antiperoxidase is added; these are linked to the antigen-bound antibody by the antirabbit IgG. The PAP complexes are then stained by incubation with a chromogenic substrate to produce a colored reaction product. Called also PAP t.
Medical dictionary.
2011.
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technique — The manner of performance, or the details, of any surgical operation, experiment, or mechanical act. SEE ALSO: method, operation, procedure. SYN: technic. [Fr., fr. G. technikos, relating to techne, art, skill] airbrasive t. a method of grinding … Medical dictionary
PAP technique — peroxidase antiperoxidase t … Medical dictionary
PAP technique — (1) Colloquial abbreviation for Papanicolaou s stain, (2) Peroxidase antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen. In the first stage the material, eg. a section, is reacted with a… … Dictionary of molecular biology
immunoperoxidase technique — a method of histologic staining in which a peroxidase labeled antibody that binds to antigen is added to tissue, and the sites of its localization are revealed by addition of a chromogenic substrate system that produces a colored reaction product … Medical dictionary
PAP — Acronym for peroxidase antiperoxidase complex. Abbreviation for 3′ phosphoadenosine 5′ phosphate. See P. technique. * * * pancreatitis associated protein; Papanicolaou [test]; papaverine; passive aggressive personality; password authentication… … Medical dictionary
pap — A food of soft consistency, like that of breadcrumbs soaked in milk or water. * * * pancreatitis associated protein; Papanicolaou [test]; papaverine; passive aggressive personality; password authentication protocol; patient assessment program;… … Medical dictionary
