catalase test

catalase test
(for the production of heat-stable catalase by bacteria) a culture is treated with hydrogen peroxide and heated. The presence of gas bubbles indicates a positive reaction. Micrococci, staphylococci, most species of Bacillus, and anaerobic diphtheroids are catalase-positive; streptococci, pneumococci, and most Actinomyces are catalase-negative. Catalase quantity and heat stability are species-dependent; inclusion of the detergent Tween-80 makes the test semiquantitative.

Semiquantitative catalase test, the column height recorded as greater than or less than 45 mm.


Medical dictionary. 2011.

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