In situ hybridization, fluorescent
An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes. "Fluorescent" means emitting light that comes from a reaction within the emitter. "In situ" refers to the fact that this techniques is done with the chromosomes, cells or tissue in place (in situ) on a microscope slide. In situ hybridization is a technique in which single-stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. Through nucleic acid hybridization, the degree of sequence identity can be determined and specific sequences detected and sometimes those sequences can be located on a specific chromosome. A nucleic acid labelled with a fluorescent dye is hybridized to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes. Fluorescent in situ hybridization is abbreviated FISH.

Medical dictionary. 2011.

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  • In situ hybridization — (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue ( in situ ), or, if the tissue is small enough (e.g. plant seeds,… …   Wikipedia

  • Fluorescent in situ hybridization — can be used to find out where the fluorescent probe bound to the chromosome. FISH is often used for finding specific features in DNA. These features can be used in genetic counseling, medicine, and species identification. Probes Probes are often… …   Wikipedia

  • Fluorescent in situ hybridization — Hybridation fluorescente in situ Pour les articles homonymes, voir FISH. Technique de l hybridation fluorescente in situ. En A : sonde. B : sonde colorée à l aide d un …   Wikipédia en Français

  • Fluorescent in situ hybridization — An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes. Fluorescent means emitting light that comes from a reaction within the… …   Medical dictionary

  • In situ hybridization — The use of a DNA or RNA probe to detect the complementary sequence. In situ hybridization is like all nucleic acid hybridization in being a technique in which single stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes …   Medical dictionary

  • Fluorescence in situ hybridization — A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the… …   Wikipedia

  • Chromogenic in situ hybridization — CISH, or chromogenic in situ hybridization, is a process in which a labeled complementary DNA or RNA strand is used to localize a specific DNA or RNA sequence in a tissue specimen. CISH methodology may be used to evaluate gene amplification, gene …   Wikipedia

  • fluorescence in situ hybridization — (FISH) in situ hybridization in which DNA probes are labeled with fluorescent tags and hybridized to metaphase or nondividing (interphase) chromosome spreads to identify and localize specific sequences. See also chromosome painting, under… …   Medical dictionary

  • fluorescence in situ hybridization — ▪ medicine       technique that employs fluorescent probes for the detection of specific deoxyribonucleic acid ( DNA) sequences in chromosomes (chromosome). FISH has a much higher rate of sensitivity and specificity than other genetic diagnostic… …   Universalium

  • Hybridization, fluorescent in situ — An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes. Fluorescent means emitting light that comes from a reaction within the… …   Medical dictionary

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