biuret reaction

biuret reaction
biuret reaction n a reaction that is shown by biuret, proteins, and most peptides on treatment in alkaline solution with copper sulfate and that results in a violet color

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formation of a chelate having an intense violet-red color when biuret or compounds having two or more adjacent peptide bonds (e.g., proteins) are reacted with copper sulfate in alkaline solution; the reaction forms the basis for a colorimetric method for detection of protein (biuret test).

Medical dictionary. 2011.

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  • biuret reaction — biureto reakcija statusas T sritis chemija apibrėžtis Baltymų ir peptidų nustatymo reakcija. atitikmenys: angl. biuret reaction rus. биуретовая реакция …   Chemijos terminų aiškinamasis žodynas

  • biuret reaction — noun : a reaction shown by biuret, proteins, and most peptides on treatment in alkaline solution with copper sulfate, resulting in a violet color, and used especially in testing for proteins …   Useful english dictionary

  • biuret test — n a test esp. for proteins using the biuret reaction * * * a colorimetric test for protein that makes use of the biuret reaction (q.v.) …   Medical dictionary

  • Biuret test — The biuret test is a chemical test used for detecting the presence of peptide bonds. In a positive test, a copper(II) ion is reduced to copper(I), which forms a complex with the nitrogens and carbons of the peptide bonds in an alkaline solution.… …   Wikipedia

  • biuret — /ˈbaɪjurɛt/ (say buyyoohret) noun an organic compound, NH2CONHCONH2, formed by heating urea. In strongly alkaline solution, it gives a violet colour when copper sulphate is added (the biuret reaction), and is therefore used as a test for protein …  

  • biuret reagent — any of various alkaline copper sulfate solutions containing a variety of stabilizers, used in the biuret reaction (q.v.) …   Medical dictionary

  • biuret — ● biuret nom masculin Diamide formé par la décomposition de l urée par la chaleur. (Le biuret donne, en milieu alcalin, avec l ion cuivrique, un complexe violet pourpre qui sert à caractériser l urée et les peptones.) ⇒BIURET, subst. masc. CHIM.… …   Encyclopédie Universelle

  • Reaction du biuret — Réaction du biuret La réaction du biuret est une réaction mettant en évidence les liaisons peptidiques. La méthode consiste à réaliser un dosage direct par photospectrométrie à 540nm, lié à la formation d un complexe coloré en mauve entre le Cu2+ …   Wikipédia en Français

  • Biuret — Général …   Wikipédia en Français

  • Biuret reagent — The Biuret reagent is made of potassium hydroxide (KOH) and copper (II) sulfate (CuSO4), together with potassium sodium tartrate (KNaC4H4O6·4H2O). The blue reagent turns violet in the presence of proteins, and changes to pink when combined with… …   Wikipedia

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