Hotis test

Ho·tis test 'hōt-əs- n a test for the presence of the common streptococcus of bovine mastitis in milk which is made by incubating a sample of milk with an aniline dye and in which the appearance of yellow patches indicates the presence of the organism

Hotis Ralph P. (1890-1935)

American agricultural marketing specialist. Hotis was an employee of the U.S. Department of Agriculture and coordinated the testing of the fitness of milk for consumption. The test method was published in 1936 and posthumously named in his honor.

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(for mastitis in cows) fresh milk containing bromcresol purple is incubated for 24 hours; a positive reaction is the formation of yellow flakes on the sides of the test tube.