isoelectric focusing

isoelectric focusing
isoelectric focusing n an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points

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electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site (or focus) at which the pH is equal to its isoelectric point. Called also electrofocusing.

Medical dictionary. 2011.

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  • Isoelectric focusing — (IEF), also known as electrofocusing, is a technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis, usually performed in a gel, that takes advantage of the fact that a molecule s… …   Wikipedia

  • isoelectric focusing — isoelectric focusing. См. изоэлектрическое фокусирование. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • isoelectric focusing — Electrophoresis in a stabilized pH gradient. High resolution method for separating molecules, especially proteins, that carry both positive and negative charges. Molecules migrate to the pH corresponding to their isoelectric point. The gradient… …   Dictionary of molecular biology

  • isoelectric focusing — noun Date: 1966 an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their… …   New Collegiate Dictionary

  • isoelectric focusing — noun : an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points …   Useful english dictionary

  • Isoelectric focusing — …   Википедия

  • isoelectric focusing — noun Biochemistry a technique of electrophoresis in which the resolution is improved by maintaining a pH gradient between the electrodes …   English new terms dictionary

  • Isoelectric point — The isoelectric point (pI) is the pH at which a particular molecule or surface carries no net electrical charge. Amphoteric molecules called zwitterions contain both positive and negative charges depending on the functional groups present in the… …   Wikipedia

  • isoelectric — Of equal electrical potential. Cf.:i. point. SYN: isopotential. i. focusing electrophoresis of small molecules or macromolecules in a pH gradient. * * * iso·elec·tric .ī sō i lek trik adj 1) having or representing the same or connecting points… …   Medical dictionary

  • IEF —    Isoelectric focusing …   Forensic science glossary

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