polymerase chain reaction

polymerase chain reaction
polymerase chain reaction n an in vitro technique for rapidly synthesizing large quantities of a given DNA segment that involves separating the DNA into its two complementary strands, binding a primer to each single strand at the end of the given DNA segment where synthesis will start, using DNA polymerase to synthesize two-stranded DNA from each single strand, and repeating the process abbr. PCR

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(PCR) a type of rapid nucleic acid amplification of specific DNA or RNA sequences, allowing small quantities of short sequences to be analyzed without cloning: oligonucleotide primers are annealed to single-stranded nucleotide sequences, which are copied by polymerase; the number of copies is geometrically amplified by repeated cycles of annealing and copying.

Medical dictionary. 2011.

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  • Polymerase Chain Reaction — Réaction en chaîne par polymérase Pour les articles homonymes, voir PCR. Diagramme des 4 premiers cycles de la PCR La réaction en chaîne par polymérase (PCR est l abréviation an …   Wikipédia en Français

  • polymerase chain reaction — n. a technique in which a known DNA sequence is synthesized at high temperatures by means of a polymerase, producing millions of copies for statistical analysis: used in DNA fingerprinting, in detecting minute quantities of cancer cells, etc. * * …   Universalium

  • polymerase chain reaction — n. a technique in which a known DNA sequence is synthesized at high temperatures by means of a polymerase, producing millions of copies for statistical analysis: used in DNA fingerprinting, in detecting minute quantities of cancer cells, etc …   English World dictionary

  • polymerase chain reaction — polymerase chain reaction. См. полимеразная цепная реакция. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… …   Wikipedia

  • Polymerase Chain Reaction — Die Polymerase Kettenreaktion (englisch Polymerase Chain Reaction, PCR) ist eine Methode, um die Erbsubstanz DNA in vitro zu vervielfältigen. Dazu wird ein Enzym verwendet, die DNA Polymerase. Der Begriff Kettenreaktion beschreibt in diesem… …   Deutsch Wikipedia

  • polymerase chain reaction — polimerazinė grandininė reakcija statusas T sritis augalininkystė apibrėžtis DNR fragmentų dauginimas in vitro. atitikmenys: angl. PCR; polymerase chain reaction rus. полимеразная цепная реакция; ПЦР ryšiai: sinonimas – PGR …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • Polymerase chain reaction optimization — The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. Contents 1… …   Wikipedia

  • polymerase chain reaction — The first practical system for in vitro amplification of DNA, and as such one of the most important recent developments in molecular biology. Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for …   Dictionary of molecular biology

  • Polymerase Chain Reaction — Po|ly|me|ra|se Chain Re|ac|ti|on [pɔ lɪməreɪz tʃeɪn rɪ ækʃn; engl. = Polymerase Kettenreaktion]: svw. ↑ PCR …   Universal-Lexikon

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