Western blot
A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). There is also a Northern blot.

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West·ern blot .wes-tərn-'blät n a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of protein for identification by a suitable molecular probe and is used esp. for the detection of antibodies compare NORTHERN BLOT, SOUTHERN BLOT
Western blot·ting -'blät-iŋ n

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[by analogy to Southern blot] a blot obtained by transferring proteins separated electrophoretically to a nitrocellulose membrane or other immobilizing matrix, followed by overlaying the matrix first with antibody against the protein of interest, then with labeled antibody against the first antibody; used for analysis and identification of specific proteins.

Medical dictionary. 2011.

Look at other dictionaries:

  • Western-Blot — Western Blot, auch Immunblot (engl. Immunoblot) bezeichnet die Übertragung (engl. Blotting) von Proteinen, die anschließend über unterschiedliche Reaktionen nachgewiesen werden können, auf eine Trägermembran. Die Übertragung kann auf… …   Deutsch Wikipedia

  • Western Blot — (Westernblot), auch Immunblot (engl. Immunoblot) bezeichnet die Übertragung (engl. Blotting) von Proteinen auf eine Trägermembran, die anschließend über unterschiedliche Reaktionen nachgewiesen werden können. Die Übertragung kann auf… …   Deutsch Wikipedia

  • Western-blot — Un transfert de protéines[1] (également appelé western blot, protein gel blot(en) ou buvardage de western), est une méthode de biologie moléculaire permettant la détection et l identification de protéines spécifiques dans un échantillon… …   Wikipédia en Français

  • Western Blot — Un transfert de protéines[1] (également appelé western blot, protein gel blot(en) ou buvardage de western), est une méthode de biologie moléculaire permettant la détection et l identification de protéines spécifiques dans un échantillon… …   Wikipédia en Français

  • Western blot — Resultado del Western blot: una membrana con las proteínas separadas en la electroforesis unidas, de las que sólo se disciernen aquellas contra las se ha añadido un anticuerpo. El Western blot, o inmunoblot, es una técnica analítica usada para… …   Wikipedia Español

  • Western blot — The western blot (alternatively, immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the… …   Wikipedia

  • Western blot — Un transfert de protéines[1] (également appelé western blot ou buvardage de western), est une méthode de biologie moléculaire permettant la détection et l identification de protéines spécifiques dans un échantillon biologique (sérum ou autre… …   Wikipédia en Français

  • Western blot — noun An analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. See Also: western blot analysis …   Wiktionary

  • western blot — noun Usage: usually capitalized W Etymology: after Southern blot (herein) : a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of protein for identification by a suitable molecular probe and is used especially for the… …   Useful english dictionary

  • Western blot — noun Etymology: after Southern blot Date: 1983 a blot consisting of a sheet of nitrocellulose or nylon that contains spots of protein for identification by a suitable molecular probe and is used especially for the detection of antibodies •… …   New Collegiate Dictionary

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