equilibrium dialysis

equilibrium dialysis
a technique used to measure antibody-hapten affinities: solutions of pure antibody and hapten are placed in two cells separated by a semipermeable membrane, and the hapten diffuses across until the free hapten concentration is the same on both sides. From the known total amounts of antibody and hapten and the measured free hapten concentration, the concentrations of free antibody and antibody-hapten complex and the dissociation constant are calculated.

Medical dictionary. 2011.

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