two-dimensional gel electrophoresis

two-dimensional gel electrophoresis
a method of gel electrophoresis used for improved separation of complex mixtures of molecules by subjecting the support medium to electrophoresis in two directions, usually at right angles to each other, such as isoelectric focusing followed by SDS–polyacrylamide gel electrophoresis.

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  • Two-dimensional gel electrophoresis — Two dimensional gel electrophoresis, abbreviated as 2 DE or 2 D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels.Basis for… …   Wikipedia

  • two-dimensional gel electrophoresis — A high resolution separation technique in which protein samples are separated by isoelectric focusing in one dimension and then laid on an SDS gel for size determined separation in the second dimension. Can resolve hundreds of components on a… …   Dictionary of molecular biology

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Temperature gradient gel electrophoresis — (TGGE) and Denaturing Gradient Gel Electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids… …   Wikipedia

  • Difference gel electrophoresis — (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two dimensional electrophoresis. After the gel electrophoresis, the gel is scanned with… …   Wikipedia

  • Electrophoresis (disambiguation) — Electrophoresis is both a phenomenon and a technique used in many scientific disciplines. Interface and Colloid ScienceElectrophoresis is the most known effect from electrokinetic phenomena. It is a motion of dispersed particles relative to a… …   Wikipedia

  • Electroforesis en gel — Gel de electroforesis. La electroforesis en gel es un grupo de técnicas empleadas por los científicos para separar moléculas basándose en propiedades como el tamaño, la forma o el punto isoeléctrico. La electroforesis en gel se utiliza… …   Wikipedia Español

  • 2D-Gel — (Autoradiogramm) 2D Gele (Coomassie gefärbt) Die zweidimensionale Gelelektrophorese oder 2D Gelelektrophorese ist eine analytische Methode in Biochemie, Mole …   Deutsch Wikipedia

  • Capillary electrophoresis — Infobox chemical analysis name = Capillary electrophoresis caption = acronym = CE classification =Electrophoresis analytes = Biomolecules Chiral molecules manufacturers = related = gel electrophoresis Two dimensional gel electrophoresis… …   Wikipedia

  • AGML — Two dimensional gel electrophoresis (2 DE) is used to separate complex protein mixtures by first separating the proteins according to their isoelectric point (pI) and then according to their molecular weight. This separation would then enable the …   Wikipedia

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