Kinyoun staining method


Kinyoun staining method
a stain for acid-fast organisms that does not require heating the stained slides. A heat-fixed smear is treated with Kinyoun carbolfuchsin. The slide is washed with water, decolorized with acid alcohol, and counterstained with methylene blue. Acid-fast organisms appear red against a blue background. Cf. Ziehl-Neelsen staining method.

Kinyoun staining method used to demonstrate acid-fast bacilli (Mycobacterium tuberculosis) in sputum smear.


Medical dictionary. 2011.

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  • Ziehl-Neelsen staining method — a stain for acid fast organisms. A heat fixed smear is flooded with Ziehl Neelsen carbolfuchsin, heated for 5 minutes, cooled, and washed. The slide is decolorized with acid alcohol, washed, and counterstained with methylene blue. Acid fast… …   Medical dictionary

  • Kinyoun carbolfuchsin solution — Kinyoun carbolfuchsin. See also Kinyoun staining method, at Table of Stains and Staining Methods, under stain …   Medical dictionary

  • Kinyoun carbolfuchsin — a modification of Ziehl Neelsen carbolfuchsin containing a higher concentration of phenol and basic fuchsin, eliminating the need for heating of the slide in staining. See Kinyoun staining method, at Stains and Staining Methods, under stain …   Medical dictionary

  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • acid-fast stain — a staining procedure for demonstrating acid fast microorganisms; see auramine rhodamine s., Fite method, Kinyoun staining method and Ziehl Neelsen staining method at Stains and Staining Methods …   Medical dictionary

  • carbolfuchsin stain — carbolfuchsin used in a stain, particularly for detection of acid fast bacteria; see Kinyoun staining method, Ziehl Neelsen staining method, and Fite method. It is also used as a counterstain for Legionella pneumophila following other routine… …   Medical dictionary

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • Acid-fast — Acid fastness is a physical property of some bacteria referring to their resistance to decolorization by acids during staining procedures.cite journal |author=Madison B |title=Application of stains in clinical microbiology |journal=Biotech… …   Wikipedia

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