fluorescent antibody technique

fluorescent antibody technique
an immunofluorescence technique in which antigen in tissue sections is located by homologous antibody labeled with fluorochrome (the single-layer technique) or by treating the antigen with unlabeled antibody followed by a second layer of labeled antiglobulin which is reactive with the unlabeled antibody (double-layer technique). Variations include direct, indirect, inhibition, and complement staining techniques.

Medical dictionary. 2011.

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