Cosmid

Cosmid
DNA from a bacterial virus into which is spliced a small fragment of a genome to be amplified and sequenced. A cosmid is an artificially constructed structure. It is used in cloning (copying) pieces of DNA. (On a technical level, a cosmid contains the cos gene of phage lambda and can be packaged in a lambda phage particle for infection into E. coli, permitting cloning of larger DNA fragments that can be introduced into bacterial hosts in plasmid vectors).
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A recombinantly engineered plasmid, a circular DNA containing, in order : a plasmid origin of replication and a drug-resistance marker, the cos (cohesive end) site from bacteriophage λ, and a fragment of eukaryotic DNA to be cloned; cosmids are constructed to permit cloning of fragments of up to about 40,000 base pairs in length, with one or more unique restriction sites being necessary to facilitate cloning.

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cos·mid 'käz-məd n a plasmid into which a short nucleotide sequence of a bacteriophage has been inserted to create a vector capable of cloning large fragments of DNA

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cos·mid (kozґmid) [cohesive end sito + plasmid] a hybrid cloning vector constructed of plasmid DNA joined together with cos sites from a bacteriophage, having advantages of both types of vector and useful for cloning large (up to 50 kb) DNA fragments.

Medical dictionary. 2011.

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Look at other dictionaries:

  • Cosmid — A cosmid, first described by Collins and Hohn in 1978, is a type of hybrid plasmid (often used as a cloning vector) that contains cos sequences, DNA sequences originally from the Lambda phage. Cosmids can be used to build genomic libraries.… …   Wikipedia

  • cosmid — cos·mid (kŏzʹmĭd) n. Genetics A hybrid vector that has been spliced with plasmid DNA for cloning large genes or gene fragments.   [Blend of cos (sequence), sequence of DNA allowing the cosmid to form as a circle (short for cohesive ends), and… …   Universalium

  • cosmid — kosmidė statusas T sritis augalininkystė apibrėžtis Plazmidė, skirta klonuoti ir dauginti dideliems DNR fragmentams. atitikmenys: angl. cosmid rus. космида …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • cosmid vector — cosmid vector. См. космида. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Cosmid — Als Cosmide bezeichnet man Plasmide, die sogenannte cos sites (cohesive site) enthalten. Dabei handelt es sich um DNA Sequenzen, die aus dem λ Phagen stammen. Diese Abschnitte liegen an den Enden des linearen Phagenchromosoms und sind jeweils von …   Deutsch Wikipedia

  • cosmid — (koz mid) A plasmid vector with lambda phage cos sites that can be packaged in a phage capsid; it is useful for cloning large DNA fragments …   Dictionary of microbiology

  • cosmid — A type of bacteriophage lambda vector. Often used for construction of genomic libraries, because of their ability to carry relatively long pieces of DNA insert, compared with plasmids …   Dictionary of molecular biology

  • cosmid — noun ˈkɑzmɪd A type of plasmid (often used as a cloning vector) constructed by the insertion of cos sequences, which are DNA sequences of the lambda phage …   Wiktionary

  • Cosmid — Cos|mid 〈n.; Gen.: s, Pl.: e; Biochemie〉 künstlich hergestellter Klonierungsträger, der das zu klonierende DNA Segment enthält u. in Plasmide von Bakterienwirten eingebaut werden kann [Etym.: <cos Gen + Plasmid] …   Lexikalische Deutsches Wörterbuch

  • cosmid — A synthetic plasmid which incorporates the cos ends, and one or more selectable markers such as an antibiotic resistance gene. Cosmids were designed as vectors able to incorporate DNA fragments up to 40 50 kb in size …   Glossary of Biotechnology

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