hybridization


hybridization
1. The process of breeding a hybrid. 2. Crossing over between related but nonallelic genes. 3. The specific reassociation of complementary strands of polynucleic acids; e.g., the formation of a DNA-RNA hybrid. 4. The process or act of forming a macromolecular hybrid in which the subunits are obtained from different sources. SYN: crossbreeding.
- cell h. fusion of two or more dissimilar cells, leading to formation of a synkaryon.
- cross h. annealing of a DNA probe to an imperfectly matching DNA molecule.
- DNA h. a technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so.
- fluorescence in situ h. SYN: fluorescent in situ h..
- fluorescent in situ h. a method used to determine the chromosomal location or expression pattern of genomic DNA or cDNA fragments. The piece of DNA to be mapped (the “probe”) is labeled with a fluorescent dye and hybridized to a chromosome preparation or to a tissue section. The probe anneals to complementary DNA or RNA sequences. Examination of the chromosomes or tissue section under a fluorescence microscope reveals the number, size, and location of the target sequences. SYN: fluorescence in situ h..
- nucleic acid h. SYN: anneal (5).
- overlap h. SYN: chromosome walking.
- in situ h. a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ h. constitutes a key step in DNA fingerprinting. SYN: in situ nucleic acid h..
- in situ nucleic acid h. SYN: in situ h..
- somatic cell h. production of a heterokaryon.

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hy·brid·iza·tion (hi″brid-ĭ-zaґshən) 1. the act or process of producing hybrids. 2. nucleic acid h. 3. in chemistry, a procedure whereby orbitals of intermediate energy and desired directional character are constructed by taking an appropriate linear combination of atomic orbitals; e.g., sp3 hybrid orbitals are formed from one s and three p orbitals.

Medical dictionary. 2011.

Look at other dictionaries:

  • Hybridization — Hy brid*i*za tion, n. The act of hybridizing, or the state of being hybridized. [1913 Webster] …   The Collaborative International Dictionary of English

  • hybridization — hybridization. См. гибридизация. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • hybridization — 1824, from hybridize (1802, from HYBRID (Cf. hybrid) + IZE (Cf. ize)) + ATION (Cf. ation) …   Etymology dictionary

  • hybridization — (Amer.) hy·brid·i·za·tion || ‚haɪbrɪdÉ™ zeɪʃn / daɪ z n. crossing of two animals or plants from different breeds or species, crossbreeding, interbreeding (also hybridisation) …   English contemporary dictionary

  • hybridization — (of nucleic acids) Technique in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity… …   Dictionary of molecular biology

  • hybridization — hibridinio grandyno sudarymas statusas T sritis radioelektronika atitikmenys: angl. hybridization vok. Hybridisierung, f rus. образование гибридной схемы, n pranc. hybridisation, f …   Radioelektronikos terminų žodynas

  • hybridization — hibridizacija statusas T sritis chemija apibrėžtis Naujo tipo orbitalių susidarymas iš kelių skirtingų orbitalių. atitikmenys: angl. hybridization rus. гибридизация …   Chemijos terminų aiškinamasis žodynas

  • hybridization — hibridizacija statusas T sritis fizika atitikmenys: angl. hybridization; hybridizing vok. Hybridisation, f; Hybridisierung, f rus. гибридизация, f pranc. hybridisation, f …   Fizikos terminų žodynas

  • hybridization — kryžminimas statusas T sritis ekologija ir aplinkotyra apibrėžtis Augalų ir gyvūnų genetikos ir selekcijos metodas – nevienodo paveldimumo individų poravimas. atitikmenys: angl. cros; crossfecundation; crossfertilization; crossing; hybridization; …   Ekologijos terminų aiškinamasis žodynas

  • hybridization — kryžminimas statusas T sritis augalininkystė apibrėžtis Dirbtinis dviejų genotipiškai skirtingų gametų sujungimas apvaisinimo metu. atitikmenys: angl. cross; hybridization rus. скрещивание …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • hybridization — kryžminimasis statusas T sritis augalininkystė apibrėžtis Natūralus dviejų genotipiškai skirtingų gametų susijungimas apvaisinimo metu. atitikmenys: angl. crossing; hybridization rus. скрещивание …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas