Hybridization, in situ
The use of a DNA or RNA probe to detect the complementary sequence. In situ hybridization is like all nucleic acid hybridization in being a technique in which single-stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. Through nucleic acid hybridization, the degree of sequence identity can be determined and specific sequences detected and sometimes those sequences can be located on a specific chromosome. In situ hybridization is done for this purpose. It involves hybridizing a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes (via fluorescent in situ hybridization, or FISH analysis).

Medical dictionary. 2011.

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  • Hybridization, fluorescent in situ — An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes. Fluorescent means emitting light that comes from a reaction within the… …   Medical dictionary

  • Hybridization, nucleic acid — A laboratory technique in which single stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences. Through nucleic acid hybridization, the degree of… …   Medical dictionary

  • Hybridization probe — In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100 1000 bases long), which is used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary… …   Wikipedia

  • hybridization — 1. The process of breeding a hybrid. 2. Crossing over between related but nonallelic genes. 3. The specific reassociation of complementary strands of polynucleic acids; e.g., the formation of a DNA RNA hybrid. 4. The process or act of forming a… …   Medical dictionary

  • hybridization — (of nucleic acids) Technique in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity… …   Dictionary of molecular biology

  • cytological hybridization — cytological hybridization. = in situ hybridization (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Nucleic acid hybridization — Hybridization is the process, discovered by Alexander Rich, of combining complementary, single stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary… …   Wikipedia

  • Fluorescence in situ hybridization — A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the… …   Wikipedia

  • In situ hybridization — (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue ( in situ ), or, if the tissue is small enough (e.g. plant seeds,… …   Wikipedia

  • Fluorescent in situ hybridization — can be used to find out where the fluorescent probe bound to the chromosome. FISH is often used for finding specific features in DNA. These features can be used in genetic counseling, medicine, and species identification. Probes Probes are often… …   Wikipedia

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