subcloning

subcloning
The process by which a DNA clone is cleaved into smaller pieces and recloned; analysis of overlapping regions of these smaller DNA fragments can confirm the entire sequence of the original DNA clone.

Medical dictionary. 2011.

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Look at other dictionaries:

  • subcloning — subcloning. См. субклонирование. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Subcloning — In molecular biology, subcloning is a technique used to move a particular gene of interest from a parent vector to a destination vector in order to further study its functionality. Subcloning is not to be confused with molecular cloning, a… …   Wikipedia

  • субклонирование — subcloning субклонирование. Получение субклонов <subclones>, входящих в состав уже клонированного сегмента большего размера. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во… …   Молекулярная биология и генетика. Толковый словарь.

  • DERB — Dual expression recombinase based (DERB) single vector system is a method of efficient cloning and subcloning of plasmid vectors for high throughput screening (HTS) and verification of protein protein interactions inside living cells. DERB was… …   Wikipedia

  • DNA end — or sticky end refers to the properties of the end of a molecule of DNA or a recombinant DNA molecule. The concept is important in molecular biology, especially in cloning or when subcloning inserts DNA into vector DNA. All the terms can also be… …   Wikipedia

  • DNA sequencing theory — is the broad body of work that attempts to lay analytical foundations for DNA sequencing. The practical aspects revolve around designing and optimizing sequencing projects (known as strategic genomics ), predicting project performance,… …   Wikipedia

  • Multiple cloning site — A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to 20) restriction sites a standard feature of engineered plasmids.[1] Restriction sites within an MCS are typically unique, occurring only …   Wikipedia

  • Nestin (protein) — Nestin Identifiers Symbols NES; FLJ21841; Nbla00170 External IDs OMIM:  …   Wikipedia

  • Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

  • GABPA — GA binding protein transcription factor, alpha subunit 60kDa, also known as GABPA, is a human gene. PBB Summary section title = summary text = This gene encodes one of three GA binding protein transcription factor subunits which functions as a… …   Wikipedia

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